LanYFP

Tetrameric yellow fluorescent protein from Branchiostoma lanceolatum, also known as blFP-Y3.

Sequence Author: Allele Biotechnology

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HomePlasmidsFluorescent Protein Genes & PlasmidsLanYFP
600 400 200 End (660) StuI (640) TaqII (625) SmlI (595) BpuEI (580) EaeI (545) BstXI (540) HaeII (498) AfeI (496) MspA1I (403) EcoP15I (340) MmeI (323) BbsI (284) BtgZI - BspEI - BsaWI (244) BciVI (241) AhdI (210) BfuAI - BspMI (200) AlwNI (192) MflI * - BstYI - BamHI (152) BaeGI - Bme1580I (135) StyI (125) BpmI (121) BseRI (118) PflMI (94) TsoI (86) BsrFI (79) PspFI (13) BseYI (9) Start (0) LanYFP LanYFP 660 bp
End  (660)
0 sites
StuI  (640)
1 site		 A G G C C T T C C G G A
TaqII  (625)
1 site		 G A C C G A ( N ) 9 N N C T G G C T ( N ) 9
Sticky ends from different TaqII sites may not be compatible.
SmlI  (595)
1 site		 C T Y R A G G A R Y T C
Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (580)
1 site		 C T T G A G ( N ) 14 N N G A A C T C ( N ) 14
Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EaeI  (545)
1 site		 Y G G C C R R C C G G Y
BstXI  (540)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
HaeII  (498)
1 site		 R G C G C Y Y C G C G R
AfeI  (496)
1 site		 A G C G C T T C G C G A
MspA1I  (403)
1 site		 C M G C K G G K C G M C
EcoP15I  (340)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
MmeI  (323)
1 site		 T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18
Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbsI  (284)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgZI  (244)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BspEI  (244)
1 site		 T C C G G A A G G C C T
BsaWI  (244)
1 site		 W C C G G W W G G C C W
Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
BciVI  (241)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
AhdI  (210)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BfuAI  (200)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (200)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AlwNI  (192)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
MflI  (152)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (152)
1 site		 R G A T C Y Y C T A G R
BamHI  (152)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BaeGI  (135)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (135)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
StyI  (125)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BpmI  (121)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BseRI  (118)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PflMI  (94)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
TsoI  (86)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (79)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (13)
1 site		 C C C A G C G G G T C G
BseYI  (9)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
Start  (0)
0 sites
LanYFP
1 .. 660  =  660 bp
219 amino acids  =  24.7 kDa
Product: tetrameric yellow fluorescent protein from Branchiostoma lanceolatum, also known as blFP-Y3
LanYFP
1 .. 660  =  660 bp
219 amino acids  =  24.7 kDa
Product: tetrameric yellow fluorescent protein from Branchiostoma lanceolatum, also known as blFP-Y3
ORF:  1 .. 660  =  660 bp
ORF:  219 amino acids  =  24.7 kDa
ORF:  109 .. 660  =  552 bp
ORF:  183 amino acids  =  19.2 kDa  (no start codon)
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ecliptic pHluorin
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mRFP1
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phKikGR1-S1
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mRuby3
phKO1-S1
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mScarlet
phmAG1-S1
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ratiometric pHluorin
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hKO
mTFP1
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rsEGFP
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rsEGFP2
hMGFP
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SBFP2
hmKO2
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SCFP3A
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SiriusGFP
hrGFP
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Skylan-NS
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smURFP
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super-ecliptic pHluorin
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superfolder GFP
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pmCherry-1
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SuperNova2
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pmCherry-N1
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Kaede
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pMFAPalpha2
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pAmCyan
pMiCy1-S1
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pmKate2-C
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pmKeima-Red-S1
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pmKikGR1-S1
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pAsRed2-N1
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