hMGFP

Monster Green® fluorescent protein (Monster GFP), a modified version of Montastrea cavernosa GFP.

Sequence Author: Promega

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HomePlasmidsFluorescent Protein Genes & PlasmidshMGFP
600 400 200 End (684) NaeI (678) BglI (677) BsrFI - NgoMIV (676) BsiHKAI (644) PflMI - DraIII (579) BsgI (577) BstXI (552) BtgZI (488) BtsI AcuI BtsαI Eco57MI (487) BsaAI (450) BanII (431) BmrI (425) HincII (378) PflFI - Tth111I (373) TatI - BsrGI (353) BssSI - BssSαI (261) AvaI - BsoBI (250) StyI (215) BbsI (192) BstEII (190) AleI (175) ApoI (64) BclI * (8) Start (0) hMGFP hMGFP 684 bp
End  (684)
0 sites
NaeI  (678)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BglI  (677)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BsrFI  (676)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
NgoMIV  (676)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BsiHKAI  (644)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
PflMI  (579)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
DraIII  (579)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsgI  (577)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstXI  (552)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BtgZI  (488)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BtsI  (487)
1 site		 G C A G T G N N C G T C A C
AcuI  (487)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BtsαI  (487)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
Eco57MI  (487)
1 site		 C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (450)
1 site		 Y A C G T R R T G C A Y
BanII  (431)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
BmrI  (425)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
HincII  (378)
1 site		 G T Y R A C C A R Y T G
PflFI  (373)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (373)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
TatI  (353)
1 site		 W G T A C W W C A T G W
BsrGI  (353)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BssSI  (261)
1 site		 C A C G A G G T G C T C
BssSαI  (261)
1 site		 C A C G A G G T G C T C
AvaI  (250)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (250)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
StyI  (215)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BbsI  (192)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BstEII  (190)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AleI  (175)
1 site		 C A C N N N N G T G G T G N N N N C A C
ApoI  (64)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
BclI  (8)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
hMGFP
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein (Monster GFP), a modified version of the GFP from Montastrea cavernosa
mammalian codon-optimized with few consensus transcription factor binding sites
hMGFP
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein (Monster GFP), a modified version of the GFP from Montastrea cavernosa
mammalian codon-optimized with few consensus transcription factor binding sites
ORF:  1 .. 684  =  684 bp
ORF:  227 amino acids  =  25.9 kDa
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hdKeima570
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ratiometric pHluorin
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hKO
mTFP1
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Kaede
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pMFAPalpha2
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pmKate2-C
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KillerRed
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