Clover

Bright green-yellow fluorescent protein derived from GFP.

Sequence Author: Addgene

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No matches
600 400 200 End (717) BsrGI (710) AclI (512) MflI * - BstYI (500) BsiEI (464) EagI (461) NmeAIII (356) KpnI (317) Acc65I (313) PfoI * (281) BssSI - BssSαI (181) BsrFI (151) BtgZI (122) SacII (93) MspA1I (92) BtgI (90) BseRI (31) Start (0) Clover Clover 717 bp
End  (717)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AclI  (512)
1 site
A A C G T T T T G C A A
MflI  (500)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
BsiEI  (464)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (461)
1 site
C G G C C G G C C G G C
NmeAIII  (356)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
KpnI  (317)
1 site
G G T A C C C C A T G G
Acc65I  (313)
1 site
G G T A C C C C A T G G
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BssSI  (181)
1 site
C A C G A G G T G C T C
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
SacII  (93)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
MspA1I  (92)
1 site
C M G C K G G K C G M C
BtgI  (90)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
Clover
1 .. 717  =  717 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: bright green-yellow fluorescent protein derived from GFP (Lam et al., 2012)
Clover
1 .. 717  =  717 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: bright green-yellow fluorescent protein derived from GFP (Lam et al., 2012)
Clover
1 .. 717  =  717 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 3:  
   7 .. 717  =  711 bp
   237 amino acids  =  26.6 kDa
Product: bright green-yellow fluorescent protein derived from GFP (Lam et al., 2012)
Clover
1 .. 717  =  717 bp
239 amino acids  =  26.8 kDa
3 segments
Product: bright green-yellow fluorescent protein derived from GFP (Lam et al., 2012)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  26.8 kDa
ORF:  3 .. 494  =  492 bp
ORF:  163 amino acids  =  18.8 kDa  (no start codon)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  24.0 kDa  (no start codon)
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