CopGFP

Green fluorescent protein 2 from the copepod Pontellina plumata, also known as ppluGFP2.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidsCopGFP
600 400 200 End (669) ApaI - BaeGI - Bme1580I (569) PspOMI - EcoO109I (565) AcuI (564) BpmI (538) HincII (510) NruI (463) AclI (443) BsiHKAI (422) BtgI (413) XcmI (411) BsaWI (398) SfcI (306) BseRI (296) SphI (229) KpnI - BmrI (194) Acc65I (190) BciVI (163) PluTI - HaeII (131) SfoI (129) NarI - BsaHI (128) KasI (127) StyI (122) AccI (118) Bpu10I (86) Start (0) CopGFP CopGFP 669 bp
End  (669)
0 sites
ApaI  (569)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BaeGI  (569)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (569)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
PspOMI  (565)
1 site		 G G G C C C C C C G G G
EcoO109I  (565)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
AcuI  (564)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (538)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
HincII  (510)
1 site		 G T Y R A C C A R Y T G
NruI  (463)
1 site		 T C G C G A A G C G C T
AclI  (443)
1 site		 A A C G T T T T G C A A
BsiHKAI  (422)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
BtgI  (413)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
XcmI  (411)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsaWI  (398)
1 site		 W C C G G W W G G C C W
Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
SfcI  (306)
1 site		 C T R Y A G G A Y R T C
Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BseRI  (296)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SphI  (229)
1 site		 G C A T G C C G T A C G
KpnI  (194)
1 site		 G G T A C C C C A T G G
BmrI  (194)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
Acc65I  (190)
1 site		 G G T A C C C C A T G G
BciVI  (163)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
PluTI  (131)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
HaeII  (131)
1 site		 R G C G C Y Y C G C G R
SfoI  (129)
1 site		 G G C G C C C C G C G G
NarI  (128)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (128)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (127)
1 site		 G G C G C C C C G C G G
StyI  (122)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
AccI  (118)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Bpu10I  (86)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Start  (0)
0 sites
CopGFP
1 .. 669  =  669 bp
222 amino acids  =  24.7 kDa
Product: green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)
CopGFP
1 .. 669  =  669 bp
222 amino acids  =  24.7 kDa
Product: green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)
ORF:  1 .. 669  =  669 bp
ORF:  222 amino acids  =  24.7 kDa
ORF:  1 .. 294  =  294 bp
ORF:  98 amino acids  =  10.3 kDa
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