pRSET-EmGFP

Vector for expressing Emerald GFP in bacteria.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsFluorescent Protein Genes & PlasmidspRSET-EmGFP
BspQI - SapI (3526) NspI (3413) AflIII - PciI (3409) PspFI (3109) BseYI (3105) AlwNI (3000) AhdI (2521) ScaI (2040) BsaHI (1981) XmnI (1921) T7 promoter XbaI (47) NdeI (87) ATG 6xHis NheI (125) BmtI (129) enterokinase site BspDI * - ClaI * (184) BamHI (191) EcoRI (197) BtgI - NcoI (207) BseRI (239) PfoI * (489) BsrGI (918) AvaI - BsoBI - PaeR7I - XhoI (929) HindIII (935) BlpI (993) EcoO109I (1020) BspEI * (1070) NgoMIV (1273) NaeI (1275) BanII (1307) BsaAI (1378) DraIII (1381) PsiI (1506) pRSET-EmGFP 3600 bp
BspQI  (3526)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (3526)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NspI  (3413)
1 site		 R C A T G Y Y G T A C R
AflIII  (3409)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (3409)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PspFI  (3109)
1 site		 C C C A G C G G G T C G
BseYI  (3105)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (3000)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AhdI  (2521)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
ScaI  (2040)
1 site		 A G T A C T T C A T G A
BsaHI  (1981)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
XmnI  (1921)
1 site		 G A A N N N N T T C C T T N N N N A A G
XbaI  (47)
1 site		 T C T A G A A G A T C T
NdeI  (87)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (125)
1 site		 G C T A G C C G A T C G
BmtI  (129)
1 site		 G C T A G C C G A T C G
BspDI  (184)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (184)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BamHI  (191)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (197)
1 site		 G A A T T C C T T A A G
BtgI  (207)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NcoI  (207)
1 site		 C C A T G G G G T A C C
BseRI  (239)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PfoI  (489)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BsrGI  (918)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AvaI  (929)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (929)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (929)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (929)
1 site		 C T C G A G G A G C T C
HindIII  (935)
1 site		 A A G C T T T T C G A A
BlpI  (993)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
EcoO109I  (1020)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
BspEI  (1070)
1 site		 T C C G G A A G G C C T
* Blocked by Dam methylation.
NgoMIV  (1273)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1275)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BanII  (1307)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
BsaAI  (1378)
1 site		 Y A C G T R R T G C A Y
DraIII  (1381)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
PsiI  (1506)
1 site		 T T A T A A A A T A T T
AmpR
1734 .. 2594  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1734 .. 1802  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1734 .. 2594  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1803 .. 2594  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1734 .. 2594  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ATG
89 .. 91  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
89 .. 91  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
6xHis
101 .. 118  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
101 .. 118  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
T7 tag
122 .. 154  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
T7 tag
122 .. 154  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
enterokinase site
167 .. 181  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
167 .. 181  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   209 .. 211  =  3 bp
   1 amino acid  =  149.2 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   212 .. 214  =  3 bp
   1 amino acid  =  117.1 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   215 .. 928  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Emerald GFP
mammalian codon-optimized
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Emerald GFP
mammalian codon-optimized
ori
2765 .. 3353  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2765 .. 3353  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1148 .. 1603  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1148 .. 1603  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
1629 .. 1733  =  105 bp
AmpR promoter
1629 .. 1733  =  105 bp
T7 terminator
1004 .. 1051  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
1004 .. 1051  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 promoter
9 .. 27  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
9 .. 27  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  187 .. 942  =  756 bp
ORF:  251 amino acids  =  29.1 kDa
ORF:  89 .. 928  =  840 bp
ORF:  279 amino acids  =  31.5 kDa
ORF:  1734 .. 2594  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2198 .. 2464  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Citrine
mEos2
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cp173Venus
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mEos4b
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pRSET-BFP
CyPet
mgfp5
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-C1
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d1EGFP
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d2ECFP
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miRFP703
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mKate2
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO
pEGFP-N2
pTagYFP-N
DsRed-Express2
mKO2
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
pTurboGFP-C
EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
mRaspberry
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EGFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
TurboRFP
mApple
pd4EGFP-N1
pmOrange2-N1
TurboYFP
mAzami-Green
pDendra2
pmPlum
UnaG
mBaoJin
pDendra2-C
pmRaspberry
Venus
mCardinal
pDendra2-N
pmStrawberry
Y-FAST
mCarmine
pDG1-S1
pmUkG1-S1
yeGFP
mCerulean
pDG3-S1
pNirFP-C
YFP
mCherry
pdKeima-Red-S1
pNirFP-N
YPet
mCherry2
pdKeima570-S1
pPA-TagRFP-C
YPet (humanized)
mCherry2C
pDsRed-Express
pPA-TagRFP-N
ZsGreen
mClavGR2
pDsRed-Express-1
pPAmCherry-C1
ZsGreen1
mClover2
pDsRed-Express-C1
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