TagCFP

Monomeric cyan fluorescent protein.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidsTagCFP
600 400 200 End (717) DraIII (654) Bpu10I (601) BsaBI * (586) BtgZI (581) PasI (561) PvuII (531) MflI * - BstYI (497) BsrGI (449) AflIII (446) MmeI (401) SacII (327) XmnI (244) BstAPI (206) BstEII (180) BsrFI (148) BsaHI (107) ApaLI (64) BmgBI (63) BseRI (28) BsrBI (5) Start (0) TagCFP TagCFP 717 bp
End  (717)
0 sites
DraIII  (654)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
Bpu10I  (601)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsaBI  (586)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtgZI  (581)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
PasI  (561)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
PvuII  (531)
1 site		 C A G C T G G T C G A C
MflI  (497)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (497)
1 site		 R G A T C Y Y C T A G R
BsrGI  (449)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AflIII  (446)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
MmeI  (401)
1 site		 T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18
Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SacII  (327)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
XmnI  (244)
1 site		 G A A N N N N T T C C T T N N N N A A G
BstAPI  (206)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BstEII  (180)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsrFI  (148)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaHI  (107)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
ApaLI  (64)
1 site		 G T G C A C C A C G T G
BmgBI  (63)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BseRI  (28)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsrBI  (5)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Start  (0)
0 sites
TagCFP
1 .. 717  =  717 bp
238 amino acids  =  26.7 kDa
Product: monomeric cyan fluorescent protein
mammalian codon-optimized
TagCFP
1 .. 717  =  717 bp
238 amino acids  =  26.7 kDa
Product: monomeric cyan fluorescent protein
mammalian codon-optimized
ORF:  1 .. 717  =  717 bp
ORF:  238 amino acids  =  26.7 kDa
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  23.7 kDa  (no start codon)
ORF:  273 .. 512  =  240 bp
ORF:  79 amino acids  =  9.3 kDa
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