iRFP682

Near-infrared fluorescent protein with an emission peak at 682 nm, engineered from a bacterial phytochrome.
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HomePlasmidsFluorescent Protein Genes & PlasmidsiRFP682
800 600 400 200 End (951) EarI (938) PflMI * - AlwNI * (918) NspI - SphI (792) EcoRV (663) BbvCI - Bpu10I (600) StuI (584) BsgI (571) ClaI * - BspDI * (555) AcuI - Eco57MI (513) SacII (464) BglI (463) BtgI (461) PstI - SbfI (428) SfcI (424) AatII (374) ZraI (372) PspFI (366) BseYI (362) SacI (353) Eco53kI (351) BclI * (330) BtgZI (307) SmaI (249) XmaI - TspMI (247) BbsI (192) BsrBI (185) PluTI (173) SfoI (171) NarI (170) KasI (169) BstXI (85) EcoP15I (63) BfuAI - BspMI (49) MflI * - BstYI - BamHI (10) Start (0) iRFP682 iRFP682 951 bp
End  (951)
0 sites
EarI  (938)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
PflMI  (918)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
AlwNI  (918)
1 site		 C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
NspI  (792)
1 site		 R C A T G Y Y G T A C R
SphI  (792)
1 site		 G C A T G C C G T A C G
EcoRV  (663)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BbvCI  (600)
1 site		 C C T C A G C G G A G T C G
Bpu10I  (600)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
StuI  (584)
1 site		 A G G C C T T C C G G A
BsgI  (571)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ClaI  (555)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BspDI  (555)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
AcuI  (513)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (513)
1 site		 C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
SacII  (464)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BglI  (463)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BtgI  (461)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
PstI  (428)
1 site		 C T G C A G G A C G T C
SbfI  (428)
1 site		 C C T G C A G G G G A C G T C C
SfcI  (424)
1 site		 C T R Y A G G A Y R T C
Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AatII  (374)
1 site		 G A C G T C C T G C A G
ZraI  (372)
1 site		 G A C G T C C T G C A G
PspFI  (366)
1 site		 C C C A G C G G G T C G
BseYI  (362)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
SacI  (353)
1 site		 G A G C T C C T C G A G
Eco53kI  (351)
1 site		 G A G C T C C T C G A G
BclI  (330)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BtgZI  (307)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
SmaI  (249)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
XmaI  (247)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
TspMI  (247)
1 site		 C C C G G G G G G C C C
BbsI  (192)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsrBI  (185)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
PluTI  (173)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (171)
1 site		 G G C G C C C C G C G G
NarI  (170)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (169)
1 site		 G G C G C C C C G C G G
BstXI  (85)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
EcoP15I  (63)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BfuAI  (49)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (49)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
MflI  (10)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (10)
1 site		 R G A T C Y Y C T A G R
BamHI  (10)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Start  (0)
0 sites
iRFP682
1 .. 951  =  951 bp
316 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an emission peak at 682 nm, engineered from a bacterial phytochrome (Shcherbakova and Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP2
iRFP682
1 .. 951  =  951 bp
316 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an emission peak at 682 nm, engineered from a bacterial phytochrome (Shcherbakova and Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP2
ORF:  1 .. 951  =  951 bp
ORF:  316 amino acids  =  34.5 kDa
ORF:  1 .. 330  =  330 bp
ORF:  110 amino acids  =  11.7 kDa
ORF:  601 .. 855  =  255 bp
ORF:  84 amino acids  =  9.5 kDa
ORF:  3 .. 311  =  309 bp
ORF:  103 amino acids  =  10.4 kDa
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Individual Sequences & Maps

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ptdTomato-C1
DsRed.T3
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pTimer
DsRed1
mNectarine
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pTimer-1
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pTurboFP602-B
dTomato
mNeptune
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E2-Crimson
mNeptune2
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pGFP
pTurboFP602-PRL
E2-Red_Green
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pGFPuv
pTurboGFP-B
EBFP
mOrange2
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EBFP2
moxGFP
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pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
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pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
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phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
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phmUkG1-S1
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GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
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HcRed1
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pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
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pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
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pmCherry-C1
SuperNova2
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pmCherry-N1
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Kaede
PAmCherry
pMFAPalpha2
TagBFP
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pAmCyan
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pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
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pmKate2-N
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pmKeima-Red-S1
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pmKikGR1-S1
TagYFP
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pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
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pd1EGFP-N1
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yeGFP
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YPet
mCherry2
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