mStayGold(J)

Bright and highly photostable monomeric green fluorescent protein created by introducing multiple mutations plus an N-terminal linker into StayGold.
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No matches
600 400 200 End (678) StuI (529) BlpI (502) PstI (491) EcoP15I (471) BmgBI (423) MslI (421) EaeI * (375) BspHI (335) AvaI - BsoBI (262) DraIII (255) XmnI (244) PspFI (230) BseYI (226) ScaI (211) PasI (180) BsaAI (141) AflIII (140) TsoI (127) BtgI (86) PvuII (54) AccI (7) Start (0) mStayGold(J) mStayGold(J) 678 bp
End  (678)
0 sites
StuI  (529)
1 site
A G G C C T T C C G G A
BlpI  (502)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
PstI  (491)
1 site
C T G C A G G A C G T C
EcoP15I  (471)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BmgBI  (423)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
MslI  (421)
1 site
C A Y N N N N R T G G T R N N N N Y A C
EaeI  (375)
1 site
Y G G C C R R C C G G Y
* Blocked by Dcm methylation.
BspHI  (335)
1 site
T C A T G A A G T A C T
AvaI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
DraIII  (255)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (244)
1 site
G A A N N N N T T C C T T N N N N A A G
PspFI  (230)
1 site
C C C A G C G G G T C G
BseYI  (226)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
ScaI  (211)
1 site
A G T A C T T C A T G A
PasI  (180)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BsaAI  (141)
1 site
Y A C G T R R T G C A Y
AflIII  (140)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
TsoI  (127)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BtgI  (86)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
PvuII  (54)
1 site
C A G C T G G T C G A C
AccI  (7)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Start  (0)
0 sites
mStayGold(J)
1 .. 678  =  678 bp
226 amino acids  =  25.6 kDa
Product: bright and highly photostable monomeric green fluorescent protein (Ando et al., 2023)
includes multiple mutations plus an N-terminal iinker sequence
mStayGold(J)
1 .. 678  =  678 bp
226 amino acids  =  25.6 kDa
Product: bright and highly photostable monomeric green fluorescent protein (Ando et al., 2023)
includes multiple mutations plus an N-terminal iinker sequence
ORF:  1 .. 678  =  678 bp
ORF:  226 amino acids  =  25.6 kDa
ORF:  1 .. 678  =  678 bp
ORF:  226 amino acids  =  23.1 kDa  (no start codon)
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Download mStayGold(J).dna file

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