TurboGFP

Improved green fluorescent protein from Pontellina plumata.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidsTurboGFP
600 400 200 End (699) BseRI (628) ApaI - BanII (589) PspOMI (585) PstI (578) NspI (543) BsaBI * (464) PaqCI (452) BsiEI (345) FseI (344) EaeI - EagI - NaeI (342) NgoMIV (340) XmnI (238) MspA1I (224) PflMI (186) BlpI (175) PluTI (152) SfoI (150) NarI - BsaHI (149) KasI (148) EcoP15I (144) EarI - SapI - BspQI (130) BsrBI (17) Start (0) TurboGFP TurboGFP 699 bp
End  (699)
0 sites
BseRI  (628)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
ApaI  (589)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BanII  (589)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
PspOMI  (585)
1 site		 G G G C C C C C C G G G
PstI  (578)
1 site		 C T G C A G G A C G T C
NspI  (543)
1 site		 R C A T G Y Y G T A C R
BsaBI  (464)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PaqCI  (452)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BsiEI  (345)
1 site		 C G R Y C G G C Y R G C
Sticky ends from different BsiEI sites may not be compatible.
FseI  (344)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
EaeI  (342)
1 site		 Y G G C C R R C C G G Y
EagI  (342)
1 site		 C G G C C G G C C G G C
NaeI  (342)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (340)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
XmnI  (238)
1 site		 G A A N N N N T T C C T T N N N N A A G
MspA1I  (224)
1 site		 C M G C K G G K C G M C
PflMI  (186)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BlpI  (175)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
PluTI  (152)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (150)
1 site		 G G C G C C C C G C G G
NarI  (149)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (149)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (148)
1 site		 G G C G C C C C G C G G
EcoP15I  (144)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
EarI  (130)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (130)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (130)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
BsrBI  (17)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Start  (0)
0 sites
TurboGFP
1 .. 699  =  699 bp
232 amino acids  =  25.7 kDa
Product: improved green fluorescent protein from Pontellina plumata (Evdokimov et al., 2006)
mammalian codon-optimized
TurboGFP
1 .. 699  =  699 bp
232 amino acids  =  25.7 kDa
Product: improved green fluorescent protein from Pontellina plumata (Evdokimov et al., 2006)
mammalian codon-optimized
ORF:  1 .. 699  =  699 bp
ORF:  232 amino acids  =  25.7 kDa
ORF:  473 .. 697  =  225 bp
ORF:  75 amino acids  =  7.8 kDa
ORF:  3 .. 464  =  462 bp
ORF:  153 amino acids  =  18.3 kDa  (no start codon)
ORF:  1 .. 699  =  699 bp
ORF:  233 amino acids  =  22.7 kDa  (no start codon)
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