Citrine

Enhanced variant of YFP.

Sequence Author: Tsien Lab

|Download SnapGene Viewer
No matches
600 400 200 End (717) BsrGI (710) MflI * BstYI (500) PfoI * (281) BssSI - BssSαI (181) BsrFI (151) BtgZI (122) BanI (36) BseRI (31) Start (0) Citrine Citrine 717 bp
End  (717)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
MflI  (500)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BssSI  (181)
1 site
C A C G A G G T G C T C
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
Citrine
1 .. 717  =  717 bp
239 amino acids  =  27.0 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: enhanced variant of YFP (Heikal et al., 2000)
mammalian codon-optimized
Citrine
1 .. 717  =  717 bp
239 amino acids  =  27.0 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced variant of YFP (Heikal et al., 2000)
mammalian codon-optimized
Citrine
1 .. 717  =  717 bp
239 amino acids  =  27.0 kDa
3 segments
   Segment 3:  
   7 .. 717  =  711 bp
   237 amino acids  =  26.8 kDa
Product: enhanced variant of YFP (Heikal et al., 2000)
mammalian codon-optimized
Citrine
1 .. 717  =  717 bp
239 amino acids  =  27.0 kDa
3 segments
Product: enhanced variant of YFP (Heikal et al., 2000)
mammalian codon-optimized
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  27.0 kDa
ORF:  3 .. 716  =  714 bp
ORF:  238 amino acids  =  27.6 kDa  (no start codon)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  24.0 kDa  (no start codon)
Click here to try SnapGene

Download Citrine.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.