iRFP713

Phytochrome-based near-infrared fluorescent protein, originally termed iRFP.

Sequence Author: Verkhusha Lab

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HomePlasmidsFluorescent Protein Genes & PlasmidsiRFP713
800 600 400 200 End (951) EarI (938) PflMI * - AlwNI * (918) NspI - SphI (792) ApoI - EcoRI (769) EcoRV (663) StuI (584) BsgI (571) SacII (464) BglI (463) BtgI (461) PstI - SbfI (428) SfcI (424) AatII (374) ZraI (372) PspFI (366) BseYI (362) SacI (353) Eco53kI (351) BclI * (330) BtgZI (307) SmaI (249) XmaI - TspMI (247) BbsI (192) BsrBI (185) PluTI (173) SfoI (171) NarI (170) KasI (169) BstXI (85) EcoP15I (63) BfuAI - BspMI (49) MflI * - BstYI - BamHI (10) Start (0) iRFP713 iRFP713 951 bp
End  (951)
0 sites
EarI  (938)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
PflMI  (918)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
AlwNI  (918)
1 site		 C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
NspI  (792)
1 site		 R C A T G Y Y G T A C R
SphI  (792)
1 site		 G C A T G C C G T A C G
ApoI  (769)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (769)
1 site		 G A A T T C C T T A A G
EcoRV  (663)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
StuI  (584)
1 site		 A G G C C T T C C G G A
BsgI  (571)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SacII  (464)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BglI  (463)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BtgI  (461)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
PstI  (428)
1 site		 C T G C A G G A C G T C
SbfI  (428)
1 site		 C C T G C A G G G G A C G T C C
SfcI  (424)
1 site		 C T R Y A G G A Y R T C
Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AatII  (374)
1 site		 G A C G T C C T G C A G
ZraI  (372)
1 site		 G A C G T C C T G C A G
PspFI  (366)
1 site		 C C C A G C G G G T C G
BseYI  (362)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
SacI  (353)
1 site		 G A G C T C C T C G A G
Eco53kI  (351)
1 site		 G A G C T C C T C G A G
BclI  (330)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BtgZI  (307)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
SmaI  (249)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
XmaI  (247)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
TspMI  (247)
1 site		 C C C G G G G G G C C C
BbsI  (192)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsrBI  (185)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
PluTI  (173)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (171)
1 site		 G G C G C C C C G C G G
NarI  (170)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (169)
1 site		 G G C G C C C C G C G G
BstXI  (85)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
EcoP15I  (63)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BfuAI  (49)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (49)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
MflI  (10)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (10)
1 site		 R G A T C Y Y C T A G R
BamHI  (10)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Start  (0)
0 sites
iRFP713
1 .. 951  =  951 bp
316 amino acids  =  34.6 kDa
Product: phytochrome-based near-infrared fluorescent protein, originally termed iRFP (Filonov et al., 2011)
name was changed to iRFP713 (Shcherbakova and Verkhusha, 2013)
iRFP713
1 .. 951  =  951 bp
316 amino acids  =  34.6 kDa
Product: phytochrome-based near-infrared fluorescent protein, originally termed iRFP (Filonov et al., 2011)
name was changed to iRFP713 (Shcherbakova and Verkhusha, 2013)
ORF:  1 .. 951  =  951 bp
ORF:  316 amino acids  =  34.6 kDa
ORF:  1 .. 330  =  330 bp
ORF:  110 amino acids  =  11.7 kDa
ORF:  601 .. 855  =  255 bp
ORF:  84 amino acids  =  9.5 kDa
ORF:  3 .. 311  =  309 bp
ORF:  103 amino acids  =  10.4 kDa
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