TurboYFP

Enhanced derivative of PhiYFP.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidsTurboYFP
600 400 200 End (732) BspEI - BsaWI (715) BssHII (672) BsaI (658) TaqII (649) BlpI (619) ApaLI (577) BssSI - BssSαI (499) EarI SapI BspQI (472) BtsI - BtsαI (435) PspFI (406) PflMI - BseYI (402) NmeAIII (347) NspI SphI (258) TsoI (254) XmnI (238) AleI (178) SgrAI (148) SacII (90) BsrDI (66) MflI * - BstYI (32) Start (0) TurboYFP TurboYFP 732 bp
End  (732)
0 sites
BspEI  (715)
1 site		 T C C G G A A G G C C T
BsaWI  (715)
1 site		 W C C G G W W G G C C W
Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
BssHII  (672)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
BsaI  (658)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
TaqII  (649)
1 site		 G A C C G A ( N ) 9 N N C T G G C T ( N ) 9
Sticky ends from different TaqII sites may not be compatible.
BlpI  (619)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
ApaLI  (577)
1 site		 G T G C A C C A C G T G
BssSI  (499)
1 site		 C A C G A G G T G C T C
BssSαI  (499)
1 site		 C A C G A G G T G C T C
EarI  (472)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (472)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (472)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
BtsI  (435)
1 site		 G C A G T G N N C G T C A C
BtsαI  (435)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
PspFI  (406)
1 site		 C C C A G C G G G T C G
PflMI  (402)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BseYI  (402)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
NmeAIII  (347)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NspI  (258)
1 site		 R C A T G Y Y G T A C R
SphI  (258)
1 site		 G C A T G C C G T A C G
TsoI  (254)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
XmnI  (238)
1 site		 G A A N N N N T T C C T T N N N N A A G
AleI  (178)
1 site		 C A C N N N N G T G G T G N N N N C A C
SgrAI  (148)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SacII  (90)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BsrDI  (66)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
MflI  (32)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (32)
1 site		 R G A T C Y Y C T A G R
Start  (0)
0 sites
TurboYFP
1 .. 732  =  732 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
TurboYFP
1 .. 732  =  732 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
ORF:  1 .. 732  =  732 bp
ORF:  243 amino acids  =  26.9 kDa
ORF:  3 .. 650  =  648 bp
ORF:  215 amino acids  =  25.3 kDa  (no start codon)
ORF:  1 .. 732  =  732 bp
ORF:  244 amino acids  =  23.9 kDa  (no start codon)
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ecliptic pHluorin
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ratiometric pHluorin
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hKO
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